MCQ on Restriction Endonuclease with Answers (PDF)

Restriction endonucleases, or restriction enzymes, are proteins that cut DNA at specific recognition sequences, usually palindromic. Found naturally in bacteria, they serve as a defense mechanism by cleaving foreign DNA, such as that of viruses. Each enzyme recognizes a particular sequence of nucleotides and cuts at or near this site, producing fragments with either sticky ends or blunt ends. These enzymes are vital tools in molecular biology for genetic engineering, cloning, and DNA analysis, enabling precise manipulation of DNA. This is an MCQ on Restriction Endonuclease with answers. You can download these questions as PDF from the download link provided in the results page of this quiz.

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1. 
Which of the following best describes the activity of a restriction enzyme?

2. 
Which of the following components is necessary for the activity of Type II restriction enzymes?

3. 
What type of sequences do most restriction enzymes recognize?

4. 
Which enzyme is responsible for sealing DNA fragments generated by restriction enzymes?

5. 
What is a key feature of Type III restriction enzymes?

6. 
What role do restriction enzymes play in gel electrophoresis analysis?

7. 
Which characteristic allows some restriction enzymes to be more effective in DNA cloning?

8. 
Which of the following is an example of a restriction enzyme that generates blunt ends?

9. 
How does methylation at a restriction enzyme's recognition site affect the enzyme's activity?

10. 
Which term describes the combination of restriction enzymes from different species that recognize the same sequence but cut differently?

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