MCQ on Southern Blotting Principles and Applications




Southern blotting is a molecular biology technique used to detect specific DNA sequences in a sample. It involves digesting DNA, electrophoresis, transferring the DNA to a membrane, and then using a labeled probe to hybridize and identify the target DNA sequence. This technique is named after its inventor, Edwin Southern. This is an MCQ on Southern Blotting Technique and its Applications.

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Learn more: MCQ on Western Blotting

1. 
What is the primary purpose of Southern blotting?

2. 
Who is the technique "Southern blotting" named after?

3. 
In Southern blotting, what is the first step in the process?

4. 
Which type of molecules is separated by size in a Southern blot?

5. 
What is the purpose of denaturing the DNA fragments in a Southern blot?

6. 
After separation by size in agarose gel electrophoresis, how are DNA fragments transferred to a solid support in Southern blotting?

7. 
What is the solid support typically used in Southern blotting to immobilize DNA fragments?

8. 
What is the purpose of the blotting step in Southern blotting?

9. 
What is the probe in Southern blotting?

10. 
What is the role of the probe in Southern blotting?

11. 
How is the target DNA sequence visualized after hybridization with the probe in Southern blotting?

12. 
Which of the following is NOT an application of Southern blotting?

13. 
Which of the following is an essential component for the hybridization step in Southern blotting?

14. 
In Southern blotting, what is the role of autoradiography or chemiluminescence detection?

15. 
Southern blotting is often used in genetic analysis. Which blotting technique is used for RNA molecules?



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Southern Blotting MCQ

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