MCQ on Next Generation Sequencing




Next-generation sequencing (NGS) encompasses advanced DNA sequencing technologies capable of high-throughput, parallel sequencing of millions of DNA fragments. This approach revolutionizes genomic research by offering rapid, cost-effective analyses, enabling various applications such as whole genome sequencing, transcriptomics, and metagenomics. This is an MCQ on Next Generation Sequencing Principle and Applications.

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1. 
What is the primary advantage of Next-Generation Sequencing (NGS) over traditional Sanger sequencing?

2. 
Which technology is commonly used in Next-Generation Sequencing (NGS) for simultaneous sequencing of millions of DNA fragments?

3. 
What role does clonal amplification play in NGS?

4. 
Which of the following is not a commonly used NGS platform?

5. 
What is the purpose of the adapter sequences in NGS library preparation?

6. 
Which step in the NGS workflow involves the hybridization of DNA fragments to a flow cell?

7. 
What is the primary function of the sequencing-by-synthesis method in NGS?

8. 
Which NGS platform utilizes reversible terminator chemistry for sequencing?

9. 
What is the significance of paired-end sequencing in NGS?

10. 
Which term describes the step where sequencing data is aligned to a reference genome in NGS analysis?

11. 
Which aspect makes NGS suitable for whole-genome sequencing projects?

12. 
Which technology is known for its ability to produce long-read sequencing data in NGS?

13. 
What is the role of bioinformatics in NGS data analysis?

14. 
Which term refers to the process of converting raw sequencing data into meaningful information, such as identifying variants or mutations?

15. 
What is the main advantage of the single-molecule sequencing approach in NGS?



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MCQ on Next Generation Sequencing

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