MCQ on SDS PAGE and Its Applications (Polyacrylamide Gel Electrophoresis)

MCQ on SDS PAGE: SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) is a widely used laboratory technique to separate proteins based on their size. It involves denaturing proteins with SDS and then subjecting them to electrophoresis through a polyacrylamide gel, enabling accurate molecular weight determination and protein analysis. This MCQ will help you to understand the concept of SDS PAGE and its applications in biological research.

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Learn more: MCQ on Electrophoresis

PAGE stands for:

PAGE is a widely used technique for the separation of:

In PAGE, smaller molecules move faster than larger ones through the gel matrix. What property of molecules determines their mobility?

What is the purpose of adding sodium dodecyl sulfate (SDS) to protein samples before running them on SDS-PAGE?

SDS-PAGE is commonly used to analyze protein samples by separating them based on their:

What is the role of a reducing agent, such as 2-mercaptoethanol, in SDS-PAGE?

What is the purpose of running a protein molecular weight marker alongside experimental samples in SDS-PAGE?

Two-dimensional gel electrophoresis (2D-PAGE) is a powerful technique that combines two separation methods. What are these two methods?

PAGE has important applications in the study of DNA, including:

What is the primary application of agarose gel electrophoresis in DNA analysis?

In capillary gel electrophoresis (CGE), DNA fragments are separated based on:

PAGE is commonly used in protein research to:

In Western blotting, proteins separated by SDS-PAGE are transferred to a membrane and probed with specific antibodies. What is the purpose of this technique?

In DNA sequencing, the separation of DNA fragments by PAGE is a crucial step. What is the common sequencing method that uses PAGE?

PAGE is an essential tool in research related to genetics, proteomics, and molecular biology. Which technique allows the visualization of proteins directly on the gel using specific dyes or stains?

Related posts:

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  2. MCQ on Protein Structure and Functions
  3. MCQ on Blotting Techniques (Types and Applications)
  4. Advanced Biochemistry MCQ-20 (LG Level)
  5. MCQ on Amino Acid Biosynthesis

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MCQ on SDS PAGE

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Related posts:

  1. MCQ on Proteins
  2. MCQ on Protein Structure and Functions
  3. MCQ on Blotting Techniques (Types and Applications)
  4. Advanced Biochemistry MCQ-20 (LG Level)
  5. MCQ on Amino Acid Biosynthesis

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