MCQ on Blotting Techniques (Types and Applications)

MCQ on Blotting Techniques: Blotting is a laboratory technique used to transfer molecules, typically DNA, RNA, or proteins, from a gel matrix (such as agarose or polyacrylamide gel) to a solid membrane. This technique allows for the detection, identification, and analysis of specific molecules through various assays like Southern, Northern, or Western blotting.

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Welcome to your MCQ on Blotting Technique

Which of the following blotting techniques is used to detect specific RNA molecules in a sample?

Southern blotting is used to detect specific DNA sequences in a sample. The technique is named after:

Which blotting technique involves the separation of proteins by gel electrophoresis and subsequent transfer to a membrane for protein detection?

Eastern blotting is a variation of which blotting technique?

Which type of membrane is commonly used in blotting techniques to capture and immobilize the target molecules?

The primary purpose of blocking in blotting techniques is to:

In Western blotting, which type of molecule is detected using specific antibodies?

Which of the following techniques is NOT commonly used for transferring molecules to the membrane in blotting?

In Southern blotting, DNA fragments are usually denatured using:

The process of detecting target molecules on the membrane using labeled probes or antibodies is referred to as:

Which of the following techniques is best suited for the detection of specific DNA sequences in a complex mixture?

The main difference between Northern blotting and Western blotting is the type of molecule being detected. In Northern blotting, the target molecules are:

Which blotting technique is most commonly used to study gene expression levels and the presence of specific mRNA molecules?

In Western blotting, the target proteins are typically visualized using:

The development of autoradiography or chemiluminescence is commonly used to visualize bands of interest in:

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  3. MCQ on Proteins
  4. MCQ on Membrane Transport
  5. MCQ on Amino Acid Biosynthesis

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MCQ on Blotting Techniques

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