MCQ on Northern Blotting: Principles and Applications







Northern blotting is a molecular biology technique used to study gene expression by detecting and analyzing specific RNA molecules in a sample. It involves electrophoresis to separate RNA fragments by size and hybridization with a labeled probe to identify target RNA sequences. This is an MCQ on Northern Blotting Technique and its Applications in Biotechnology and Molecular Biology Research.

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Learn more: MCQ on Southern Blotting

1. 
What is the primary purpose of Northern blotting?

2. 
Which molecular biologist is the technique "Northern blotting" named after?

3. 
In Northern blotting, what is the first step in the process?

4. 
Which type of molecules is separated by size in a Northern blot?

5. 
After separation by size in agarose gel electrophoresis, how are RNA fragments transferred to a solid support in Northern blotting?

6. 
What is the solid support typically used in Northern blotting to immobilize RNA fragments?

7. 
What is the purpose of the blotting step in Northern blotting?

8. 
What is the probe in Northern blotting?

9. 
What is the role of the probe in Northern blotting?

10. 
How is the target RNA sequence visualized after hybridization with the probe in Northern blotting?

11. 
Which of the following is NOT an application of Northern blotting?

12. 
Which of the following is an essential component for the hybridization step in Northern blotting?

13. 
In Northern blotting, what is the role of autoradiography or chemiluminescence detection?

14. 
Which of the following is true regarding RNA denaturation in Northern blotting?

15. 
Northern blotting is often used to study gene expression. Which blotting technique is used for DNA molecules?

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MCQ on Northern Blotting




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