MCQ on Pyrosequencing of DNA




Pyrosequencing is a DNA sequencing technique that relies on the detection of released pyrophosphate upon nucleotide incorporation. It involves a series of enzymatic reactions, generating light proportional to the number of incorporated nucleotides, allowing for accurate sequence determination. This is an MCQ on Pyrosequencing of DNA- Principles and Applications.

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1. 
Pyrosequencing is based on the detection of:

2. 
What enzyme is primarily used in pyrosequencing to generate light signals during nucleotide incorporation?

3. 
Which type of nucleotide is released and generates a light signal in pyrosequencing when incorporated into the growing DNA strand?

4. 
How is the release of pyrophosphate (PPi) detected in pyrosequencing?

5. 
Which sequencing technology involves the use of a cascade of enzymatic reactions to sequence DNA?

6. 
In pyrosequencing, which nucleotide is added one at a time and the resulting light signal is measured after each addition?

7. 
Which property of the light signal is used to determine the sequence of DNA in pyrosequencing?

8. 
What is the role of the apyrase enzyme in pyrosequencing?

9. 
Which term describes the step where a nucleotide is added to the DNA template and the resulting light signal is detected in pyrosequencing?

10. 
Pyrosequencing utilizes which type of DNA template for sequencing?

11. 
Which aspect of pyrosequencing contributes to its suitability for detecting single nucleotide polymorphisms (SNPs)?

12. 
Which characteristic differentiates pyrosequencing from Sanger sequencing in terms of sequence determination?

13. 
What is the significance of the dispensation order of nucleotides in pyrosequencing?

14. 
Which step is essential before initiating the pyrosequencing reaction?

15. 
What is the primary advantage of pyrosequencing in terms of real-time monitoring?



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MCQ on Pyrosequencing

Pyrosequencing questions and answers

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