MCQ on Dark Field Microscopy with Answers for Students and Exams

Dark field microscopy is a technique that enhances contrast by using a special condenser to block direct light. Only scattered light from the specimen enters the objective lens, making the sample appear bright against a dark background. It is especially useful for observing transparent, unstained, and live specimens. This method helps in studying fine structures like bacteria, spirochetes, and thin cell components. This is an MCQ on Dark Field Microscopy and its Applications with Answers.

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Learn more: MCQ on Confocal Microscope

1. 
What is the primary purpose of using a dark field microscope?

2. 
How is the illumination in a dark field microscope different from that in a bright field microscope?

3. 
In dark field microscopy, what is the function of the condenser?

4. 
What type of specimens are best observed using dark field microscopy?

5. 
Which component of the dark field microscope is specifically designed to create the dark field illumination?

6. 
What is the main limitation of dark field microscopy?

7. 
In dark field microscopy, what effect does the aperture of the dark field condenser have on the image?

8. 
What is the key visual feature of specimens observed under dark field microscopy?

9. 
Why might dark field microscopy be preferred over bright field microscopy for certain applications?

10. 
How does the dark field microscope handle specimens that are not perfectly transparent?

11. 
What optical effect does the dark field condenser achieve to enhance image contrast?

12. 
What is a common application of dark field microscopy in microbiology?

13. 
What adjustment is typically made to the light source in a dark field microscope?

14. 
What type of specimen is least suitable for dark field microscopy?

15. 
How does dark field microscopy compare to phase contrast microscopy in terms of contrast enhancement?

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mcq on dark field microscopy

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