MCQ on Cre-Lox Recombination: Principles and Applications

Cre-lox recombination is a genetic engineering technique used to manipulate DNA in living organisms. It involves the enzyme Cre recombinase, which recognizes specific DNA sequences (loxP sites) to excise, insert, or invert genetic segments. It is valuable for conditional gene knockout and controlled gene expression in research and biotechnology. This is an MCQ on Cre lox Recombination Principles and Applications.

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1. 
What is the primary purpose of the Cre-lox recombination system in genetic research?

2. 
In the Cre-lox system, what is "Cre"?

3. 
What is the function of the "lox" sequences in the Cre-lox system?

4. 
What type of DNA recombination occurs when Cre recognizes "lox" sites and catalyzes DNA strand exchange?

5. 
In which organism was the Cre-lox system first discovered and characterized?

6. 
What is the key advantage of the Cre-lox system for conditional gene knockout experiments?

7. 
Which step is necessary for the activation of Cre recombinase in the Cre-lox system?

8. 
What term is used to describe the removal of a DNA segment between two "lox" sites by Cre recombinase?

9. 
What happens when Cre recombinase recognizes two "lox" sites in the same orientation?

10. 
What term is used to describe the recombination outcome when "lox" sites are recognized in a head-to-tail orientation?

11. 
In what type of experiment is the Cre-lox system particularly useful for studying gene function?

12. 
What is the main limitation of the Cre-lox system when used for gene knockout in mammalian cells?

13. 
Which technique is often used to achieve tissue-specific gene knockout in animals using the Cre-lox system?

14. 
What is the primary application of the Cre-lox system in mice and other model organisms?

15. 
What is the role of "loxP" sites in the Cre-lox system?

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MCQ on Cre Lox Recombination




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