Genetic Engineering MCQ (Advanced)

A 6.4 Kb plasmid DNA has two restriction endonuclease sites, HindIII and EcoRI. Complete double digestion of the plasmid with both the enzymes yields two fragments of 3.1 and 3.3 Kb. In order to study DNA repair process, a G:T mismatch was introduced in one strand of HindIII site and the damaged plasmid was incubated in a reconstituted repair system containing all the factors and enzymes required for repair. If the efficiency of the repair system is 50%, which one of the following band patterns on agarose gel will be obtained after treating the repaired plasmid with both HindIII and EcoRI? (CSIR_2015_I)

Combination of molecular markers with their classification based on either dominant or co-dominant types are shown below:

(A). SSR and RFLP: Co-dominant
(B). SSR and RAPD: Co-dominant
(C). RAPD and RFLP: Dominant
(D). AFLP and RAPD: Dominant

Which one of the following is the correct combination? (CSIR_2015_I)

Application of gene therapy in clinical trials did NOT succeed due to: (CSIR_2015_II)

A gene expressing a 50 kDa protein from a eukaryote was cloned in an E. coli plasmid under the lac promoter and operator. Upon addition of IPTG, the 50 kDa protein was not detected. Which one of the following explains the above observation? (CSIR_2015_II)

Neomycin phosphortransferase gene, frequently used as a selection marker during plant trans- formation, inactivates which one of the following antibiotics? (CSIR_2015_II)

A series of cell lines was created by fusing mouse and human somatic cells. In mouse-human somatic cell hybrids, human chromosomes tend to get lost before becoming a stable cell line. Some hybrid cell lines may carry human chromosome deletions. Each cell line was examined for the presence of chromosomes and for the production of an enzyme. The following results were obtained:

 Which segment of the chromosome has the gene encoding for the enzyme? (CSIR_2015_I)

Which one of the following techniques will you use to identify more than 1000 differentially expressed genes in normal and tumor tissues in one single experiment? (CSIR_2015_II)

The following statements are related to plant tissue culture

(A). Friable callus provides the inoculum to form cell-suspension cultures.
(B). The process known as ‘habituation’ refers to the property of callus loosing the requirement of auxin and/or cytokinin during long term culture.
(C). Cellulase and pectinase enzymes are usually used for generating protoplast cultures.
(D). During somatic embryo development, torpedo stage embryo is formed before heart stage embryo.

Which one of the following combinations of above statements is correct? (CSIR_2015_II)

A student noted the following points regarding Agrobacterium tumefaciens:

(A). A. tumefaciens is a gram-negative soil bacterium.
(B). Opine catabolism genes are present in T- DNA region of Ti-plasmid.
(C). Opines are synthesized by condensation of amino acids and α-ketoacids or amino acids and sugars.
(D). A callus culture of crown gall tissue casued by A. tumefaciens in plants can be multiplied without adding phytohormones.

Which one of the combinations of above statements is correct? (CSIR_2015_II)

Some of the following transgenic approaches could be used for functional characterization of endogenous genes in plants:

(A). Transformation using a binary vector containing a strong enhancer element and lacking the right border of T-DNA
(B). Transformation using a binary vector containing a promoter-less reporter gene sequence and a selction maker gene cassette within the T-DNA.
(C). Transformation using a binary vector containing only a strong enhancer element and a selection marker gene cassette within the T-DNA.
(D). Transformation using a binary vector lacking a reporter gene as well as both the left and right borders of T-DNA.

Which one of the following combinations can be used? (CSIR_2017_II)