MCQ on Green Fluorescent Protein (GFP)







Green fluorescent proteins (GFP) are naturally occurring proteins that emit green light when exposed to ultraviolet or blue light. Widely used as a biomarker, GFP can be genetically fused to other proteins to visualize and track their location and movement within living cells, aiding in biological research and imaging. This is an MCQ on Green Fluorescent Protein and its Applications in Biological Research.

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Learn more: MCQ on Phase Contrast Microscopy

1. 
What is the primary function of Green Fluorescent Protein (GFP)?

2. 
In what organism was GFP originally discovered and isolated?

3. 
What is the source of the green fluorescence in GFP?

4. 
How does GFP's fluorescence work?

5. 
Which scientist first cloned the GFP gene and demonstrated its utility as a genetic marker?

6. 
What is the key advantage of using GFP as a marker in molecular and cell biology studies?

7. 
In the jellyfish Aequorea victoria, which part of the GFP structure is responsible for its green fluorescence?

8. 
Which amino acid in the GFP structure is essential for the formation of the chromophore and green fluorescence?

9. 
How is GFP typically introduced into cells or organisms for labeling and visualization?

10. 
Which of the following applications is NOT associated with GFP technology?

11. 
In fluorescence microscopy, what color of light is used to excite GFP?

12. 
What is the most common wild-type (natural) form of GFP?

13. 
Which modification of GFP led to the development of the EGFP variant with improved fluorescence and stability?

14. 
How has GFP technology been used in environmental and ecological studies?

15. 
What is the primary advantage of using red-shifted variants of GFP in research?

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MCQ on Green Fluorescent Protein




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