Pairwise sequence alignment compares two sequences to identify similarities and differences, crucial in understanding evolutionary relationships and functional similarities. Algorithms like Smith-Waterman and BLAST are […]
Multiple Sequence Alignment (MSA) is the process of aligning three or more biological sequences simultaneously. It aids in identifying conserved regions, understanding evolutionary relationships, and […]
The Human Genome Project (HGP) was an international effort to map and sequence the entire human genome. Completed in 2003, it provided a reference sequence […]
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It provides crucial insights into genetic makeup, aiding in […]
Next-generation sequencing (NGS) encompasses advanced DNA sequencing technologies capable of high-throughput, parallel sequencing of millions of DNA fragments. This approach revolutionizes genomic research by offering […]
Shotgun sequencing is a method to sequence DNA by randomly breaking it into smaller fragments, sequencing them, and then assembling the overlapping sequences. This approach […]
Pyrosequencing is a DNA sequencing technique that relies on the detection of released pyrophosphate upon nucleotide incorporation. It involves a series of enzymatic reactions, generating […]
Illumina sequencing, a widely used method, employs fluorescently labeled nucleotides and reversible termination to sequence DNA fragments. Through iterative cycles of nucleotide incorporation and imaging, […]
Chargaff’s rule states that in DNA, the amount of adenine (A) equals thymine (T), and the amount of cytosine (C) equals guanine (G). This principle […]