MCQ on HPLC Instrumentation with Answers (PDF)







High-Performance Liquid Chromatography (HPL instrumentation includes a solvent reservoir, pump, injector, column, detector, and data system. The pump delivers the mobile phase through the column, where sample components separate. The detector measures the separated components, converting signals into data for analysis. HPLC is crucial in chemical, pharmaceutical, and biological research. This is an MCQ on HPLC Instrumentation with Answers. You can download the questions as PDF from the link provide in the result page of the quiz

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1. 
Which component of the HPLC system is responsible for generating the high pressure required for the separation process?

2. 
In HPLC, which type of detector is most commonly used for detecting compounds that absorb UV light?

3. 
Which of the following materials is most commonly used as the stationary phase in a reversed-phase HPLC column?

4. 
What is the primary role of the injector in an HPLC system?

5. 
Which parameter in HPLC is most directly controlled by the column thermostat?

6. 
The resolution in an HPLC separation can be improved by:

7. 
In gradient elution HPLC, what happens to the composition of the mobile phase during the run?

8. 
Which type of pump is most commonly used in modern HPLC systems?

9. 
What is the main function of the guard column in an HPLC system?

10. 
What is the purpose of using a gradient elution in HPLC?

11. 
In HPLC, what is the effect of increasing the flow rate of the mobile phase?

12. 
Which component of the HPLC system is used to separate the different analytes in the sample?

13. 
Which type of HPLC detector is most sensitive for detecting compounds that fluoresce?

14. 
What is the purpose of degassing the mobile phase in HPLC?

15. 
In HPLC, what is the purpose of the autosampler?

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