MCQ on HPLC (High Performance Lipid Chromatography) with Answers (PDF)







High-Performance Liquid Chromatography (HPLC) is an advanced analytical technique used to separate, identify, and quantify components in a mixture. HPLC involves a liquid mobile phase that carries the sample through a column packed with a stationary phase. Components in the sample interact differently with the stationary phase, leading to their separation. A detector measures the separated components as they elute from the column, and the resulting data is used for analysis. HPLC is widely used in pharmaceuticals, environmental testing, food analysis, and biochemistry due to its precision, speed, and ability to handle complex mixtures. This is an MCQ on HPLC with Answers. You can download the questions as PDF from the download link provided in the result page of this quiz.

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1. 
What is the primary function of the pump in an HPLC system?

2. 
Which component of the HPLC system is responsible for the separation of compounds?

3. 
What type of detector is most commonly used in reverse-phase HPLC for detecting non-volatile organic compounds?

4. 
In reverse-phase HPLC, which type of stationary phase is typically used?

5. 
What is the purpose of the gradient elution in HPLC?

6. 
What is the role of the injector in an HPLC system?

7. 
Which type of chromatography is characterized by the stationary phase being non-polar and the mobile phase being polar?

8. 
What is the primary advantage of using a C18 column in reverse-phase HPLC?

9. 
In HPLC, what does the term "dead volume" refer to?

10. 
What effect does increasing the mobile phase flow rate typically have on the separation in HPLC?

11. 
Which type of detector is most suitable for detecting compounds that do not have UV absorbance in HPLC?

12. 
What is the primary principle behind ion-pair chromatography used in HPLC?

13. 
In HPLC, what is the purpose of using a pre-column guard?

14. 
Which of the following factors is least likely to affect the retention time of an analyte in HPLC?

15. 
In what scenario would you use a silica-based stationary phase in HPLC?

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