MCQ on DNA Ligase Enzyme with Answers (PDF)

DNA ligase is an essential enzyme that facilitates the joining of DNA strands by catalyzing the formation of phosphodiester bonds between adjacent nucleotides. It plays a critical role in DNA replication and repair by sealing breaks or “nicks” in the sugar-phosphate backbone, particularly on the lagging strand where Okazaki fragments are formed. DNA ligase ensures the continuity of the DNA molecule, maintaining its structural integrity during cellular processes like replication, recombination, and repair. This is an MCQ on DNA Ligase Enzyme with Answers. You can download these questions as PDF from the download link provided in the results page of this quiz.

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Welcome to your MCQ on DNA Ligase

1. 
What is the primary function of DNA ligase in cellular processes?

2. 
During which cellular process does DNA ligase play a critical role in joining Okazaki fragments?

3. 
Which of the following DNA ligase types is commonly used in molecular cloning?

4. 
DNA ligase requires which of the following molecules to perform its enzymatic function?

5. 
What type of bond does DNA ligase form between adjacent nucleotides?

6. 
How does the reaction mechanism of DNA ligase involve the formation of an intermediate complex?

7. 
Which enzyme class does DNA ligase belong to?

8. 
In which type of DNA repair mechanism does DNA ligase play a critical role?

9. 
Which of the following is a common application of DNA ligase in biotechnology?

10. 
What is the most important feature of DNA ligase's substrate specificity?

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