MCQ on Cloning with Answers (PDF)

MCQ on Cloning: Gene cloning is a molecular biology technique used to create identical copies of a specific gene. It involves isolating a gene of interest and inserting it into a vector, typically a plasmid, which is a small circular DNA molecule found in bacteria. This recombinant DNA is then introduced into a host organism, usually bacteria, where it can replicate, producing many copies of the gene.

The process has several steps: (1) isolating the gene, (2) inserting it into a vector, (3) introducing the vector into host cells, and (4) selecting and growing the cells that have successfully incorporated the gene. Cloned genes can be used for various purposes, including research, the production of proteins (e.g., insulin), and the development of genetically modified organisms. Gene cloning is a fundamental tool in genetic engineering, allowing scientists to study genes, understand genetic diseases, and develop new therapies. This is an MCQ on Cloning of Genes with answers. You can download the questions as PDF from the result page of this quiz.

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1. 
Which of the following is the main enzyme used in the process of gene cloning to join DNA fragments?

2. 
Which type of vector is commonly used for cloning large DNA fragments?

3. 
What is the first step in the process of gene cloning?

4. 
Which method is used to introduce recombinant DNA into bacterial cells?

5. 
What is the purpose of antibiotic resistance genes in cloning vectors?

6. 
Which of the following is a selectable marker used in cloning?

7. 
Which type of restriction enzymes are used in gene cloning for cutting DNA at specific sites?

8. 
What is the role of a cloning vector in gene cloning?

9. 
Which technique is often used to confirm the presence of a cloned gene in host cells?

10. 
Which of the following best describes "sticky ends" in DNA cloning?

11. 
Which host organism is most commonly used in gene cloning?

12. 
The term "recombinant DNA" refers to:

13. 
What is the function of the multiple cloning site (MCS) in a cloning vector?

14. 
Which technique would you use to separate DNA fragments based on size during gene cloning?

15. 
Which of the following is a feature of plasmids that makes them suitable cloning vectors?

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