Biophysics MCQ for CSIR NET

A polymer is synthesized from an achiral amino acid. Conformation of the polymer can be investigated by the following techniques. (A). Fibre diffraction (B). Nuclear magnetic resonance spectroscopy (C). Circular dichroism spectroscopy (D). Differential scanning calorimetry Choose the combination which would indicate that the polymer adopts a helical conformation.

Match the technique with its appropriate application/use from the list of options given below.

To achieve a best resolution using a fluorescence microscope, what combination of wavelength of emitted light (λ), refractive index and the angle (2θ) by which light enters into the microscope would be the best choice for the user:

Given below are spatial and temporal techniques (column I) used to detect brain activity (column II)

Diagnosis of influenza virus infections can be done using some of the following techniques: (A). Western blot and Southern blot (B). Northern blot and western blot (C). ELISA and RT-PCR (D). PCR and electron microscopy Choose the combination of techniques that correctly lists the detection methods.

Which among the following is the simplest method to estimate the concentration of glycerol in an aqueous solution of glycerol?

From the following statements: (A) Coloured images can be obtained by transmission electron microscopy by fluorescent labeling of the specimen (B) Scanning electron microscopy requires sectioning of the sample (C) Confocal microscopy uses optical methods to obtain images from a specific focal plane and excludes light from other planes (D) Differential-interference microscopy relies on interference between polarized light due to differences in the refractive index of the object and surrounding medium (E) Visualization in epifluorescence microscopy requires staining by heavy metal atoms Choose the combination with two correct and one incorrect statements.

For identification of three proteins moving together (as a single band) upon loading in a single lane of a SDS-PAGE gel, the best method is:

Which isotope below is best suited for metabolic labelling of glyceraldehyde-3- phospho-dehydrogenase?

As cancer progresses, several genome rearrangements including translocation, deletion, duplications etc. occur. If these rearrangements are to be identified, which of the following techniques would be most suitable?