MCQ on ELISA (Enzyme Linked Immuno Sorbent Assay)







Enzyme-Linked Immunosorbent Assay (ELISA) is a biochemical test used to detect and quantify specific proteins, antibodies, or antigens in biological samples. It involves using antibodies and enzymes to create a color change that indicates the presence and concentration of the target molecule, making it valuable in various research and diagnostic applications. This an MCQ on ELISA Principles and Applications in Biological Research

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1. 
What does the acronym "ELISA" stand for?

2. 
Which component of an ELISA is immobilized on a solid surface to capture specific antigens?

3. 
What is the purpose of the "blocking" step in an ELISA?

4. 
In a direct ELISA, what directly binds to the target antigen?

5. 
What is the name of the enzyme commonly used in ELISA for signal generation?

6. 
In a competitive ELISA, what competes with the sample antigen for binding to the antibody?

7. 
What type of ELISA is often used for detecting the presence of antibodies in a patient's blood?

8. 
Which color change is typically observed when the enzyme substrate reacts in a positive ELISA result?

9. 
What is the purpose of the wash step in ELISA?

10. 
What is the primary advantage of using ELISA in diagnostics?

11. 
In a typical ELISA, what serves as the positive control to ensure the assay is working correctly?

12. 
Which type of ELISA is commonly used to measure the concentration of specific proteins, such as cytokines or hormones, in a sample?

13. 
What is the role of the enzyme-substrate reaction in ELISA?

14. 
Which of the following is NOT a common application of ELISA?

15. 
What does a high optical density (OD) value in an ELISA result indicate?

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MCQ on ELISA




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