Microscopy is one of the most powerful tools in biology, allowing us to visualize structures invisible to the naked eye. For undergraduate and postgraduate students, understanding microscopy techniques is essential for both coursework and research. Among these methods, dark field microscopy is particularly fascinating because it reveals fine details in […]
An electron microscope uses a beam of electrons instead of light to magnify specimens. It provides very high resolution, allowing scientists to see structures at the molecular or atomic level. There are two main types: transmission electron microscope (TEM) and scanning electron microscope (SEM). It is widely used in biology, […]
Dark field microscopy is a technique that enhances contrast by using a special condenser to block direct light. Only scattered light from the specimen enters the objective lens, making the sample appear bright against a dark background. It is especially useful for observing transparent, unstained, and live specimens. This method […]
A confocal microscope is an advanced optical microscope that uses laser light to scan specimens point by point. It employs a pinhole aperture to block out-of-focus light, producing clearer and sharper images. This technique allows for optical sectioning, enabling 3D reconstruction of samples. Confocal microscopes are widely used in cell […]
A compound microscope is an optical instrument that uses two sets of lenses, the objective and the eyepiece, to magnify small specimens for detailed observation. It provides higher magnification and resolution compared to a simple microscope, making it useful in biology, medicine, and research. The objective lens forms an enlarged […]