Pyrosequencing is a widely used DNA sequencing technique that relies on detecting pyrophosphate (PPi) release during DNA synthesis. This method is different from the common chain termination approach as us ed in Sanger sequencing. Pyrosequencing offers real-time sequencing and is especially useful for shorter DNA fragments. It is very useful […]
MCQ on Cloning: Gene cloning is a molecular biology technique used to create identical copies of a specific gene. It involves isolating a gene of interest and inserting it into a vector, typically a plasmid, which is a small circular DNA molecule found in bacteria. This recombinant DNA is then […]
Cloning vectors are DNA molecules used to carry and replicate a specific gene or DNA fragment in a host cell. They are essential tools in gene cloning. Common types include plasmids, bacteriophages, and cosmids. Vectors have features like an origin of replication (to multiply within the host), selectable markers (for […]
RNA interference (RNAi) is a natural biological process where small RNA molecules inhibit gene expression by degrading or blocking the translation of specific messenger RNA (mRNA). It helps regulate gene activity and defends against viral infections in cells. The process involves two main types of small RNA: small interfering RNA […]
Meiosis is a specialized form of cell division that occurs in sexually reproducing organisms to produce gametes (sperm and eggs). Unlike mitosis, which results in two genetically identical diploid cells, meiosis produces four genetically distinct haploid cells. This reduction in chromosome number is critical for maintaining the species’ chromosome count […]