Questions on Chargaff Rule (MCQ PDF

Chargaff’s rule is a key principle in molecular biology, stating that in DNA, the proportion of adenine (A) always equals that of thymine (T), and guanine (G) always equals cytosine (C). This A=T and G=C ratio varies between species but remains constant within an organism. This discovery helped Watson and Crick deduce the double-helix structure of DNA, showing how the complementary base pairs form hydrogen bonds, maintaining the stability and replication mechanism of the DNA molecule. This is article is about MCQ Questions on Chargaff Rule with answers. You can download this question as PDF from the download link provided in the results page of this quiz.

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Welcome to your MCQ on Chargaff's Rule

1. 
Chargaff's rule states that in a double-stranded DNA molecule, the ratio of purines to pyrimidines is:

2. 
If the DNA of a species has 30% guanine, what is the expected percentage of cytosine?

3. 
Which of the following best explains why Chargaff's rule does not apply to single-stranded RNA?

4. 
The fact that Chargaff's rule is universally applicable across species suggests that:

5. 
If the DNA of a bacterium has 35% adenine, what would be the percentage of guanine?

6. 
Which observation led Chargaff to conclude that DNA base composition varies between species?

7. 
Which of the following statements about Chargaff’s rule is incorrect?

8. 
In the context of Chargaff’s rule, why do organisms with higher G+C content have more stable DNA?

9. 
If a researcher finds that a sample of DNA contains 40% cytosine, what is the expected percentage of adenine in this sample?

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