MCQ on PCR and its Applications (Polymerase Chain Reaction)

MCQ on PCR and its Applications: PCR (Polymerase Chain Reaction) is a molecular biology technique used to amplify DNA segments exponentially. It involves cycles of heating and cooling to replicate a target DNA region, making it a valuable tool in DNA analysis, genetic research, and diagnostics, allowing the detection of specific sequences. This MCQ will help you to understand the concept of Polymerase Chain Reaction and its Applications in biological research.

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PCR stands for:

PCR is a technique used to amplify specific regions of DNA. Which enzyme is primarily responsible for DNA amplification in PCR?

Which step in the PCR cycle is responsible for denaturing the DNA, separating the two strands?

The initial step in a PCR cycle typically involves heating the reaction to a high temperature. What is the purpose of this step?

During which step in the PCR cycle do primers anneal to the DNA template?

What is the typical temperature range used for the annealing step in a PCR cycle?

The extension step in a PCR cycle is when DNA polymerase adds nucleotides to the 3' end of the primers. What is the optimal temperature for this step?

How many cycles of denaturation, annealing, and extension are typically performed in a standard PCR protocol?

What is the expected result after one complete PCR cycle?

What is the purpose of using a DNA template in a PCR reaction?

In real-time PCR (qPCR), the fluorescence signal is measured during:

Which component of the PCR reaction is used to facilitate the annealing of primers to the DNA template?

What is the critical temperature at which the DNA strands are separated during the denaturation step?

Which of the following is an essential part of the PCR reaction mixture, serving as a template for DNA amplification?

PCR is widely used in various applications, such as DNA sequencing, genetic testing, and molecular diagnostics. What is the primary advantage of PCR over traditional DNA cloning methods?

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mcq on pcr

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