MCQ on Column Chromatography with Answers (PDF)

Column chromatography is a technique used to separate and purify individual compounds from a mixture. It involves a column filled with a stationary phase (usually silica gel or alumina) through which the mixture is passed along with a mobile phase (a solvent or solvent mixture). As the mixture moves through the column, different components interact differently with the stationary phase, causing them to travel at different speeds. This results in the separation of components, which can be collected as they exit the column at different times. Column chromatography is widely used in organic chemistry and biochemistry for isolating and purifying substances. This is an MCQ on Column Chromatography with Answers. You can download the questions as PDF from the download link provided in the result page of this quiz.

Biophysics Notes  |  Biophysics PPTs  |  Biophysics MCQs

1. 
In column chromatography, which factor primarily determines the separation of compounds?

2. 
Which of the following stationary phases would be most suitable for separating polar compounds in normal-phase column chromatography?

3. 
In reverse-phase column chromatography, which of the following is typically the mobile phase?

4. 
Which of the following factors is most likely to cause band broadening in column chromatography?

5. 
Which method is commonly used to pack the stationary phase in a chromatography column?

6. 
In column chromatography, what is the purpose of using a gradient elution?

7. 
The term "capacity factor" (k') in column chromatography refers to:

8. 
Which type of column chromatography is specifically used to separate proteins based on their size?

9. 
Which of the following can increase the efficiency of separation in column chromatography?

10. 
What role does the mobile phase play in column chromatography?

11. 
In normal-phase chromatography, what is the effect of increasing the polarity of the mobile phase?

12. 
Why is temperature control important in column chromatography?

13. 
Which of the following detectors is commonly used in conjunction with column chromatography for detecting non-UV absorbing compounds?

14. 
What is the purpose of using a guard column in column chromatography?

15. 
In column chromatography, which of the following best describes the "theoretical plate"?

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