MCQ on Chromatography with Answers (PDF)







Chromatography is a laboratory technique used to separate, identify, and analyze components in a mixture. It involves a stationary phase and a mobile phase. As the mixture moves through the stationary phase, different components travel at different rates, leading to separation. Types include gas, liquid, paper, and thin-layer chromatography, each with specific applications. This is an MCQ on Chromatography with Answers. You can Download the questions as PDF from the download link provided in the result page of this quiz.

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1. 
Which of the following best describes the principle of chromatography?

2. 
In liquid chromatography, which of the following factors can most significantly affect the resolution between two closely eluting peaks?

3. 
Which of the following detectors is most suitable for detecting non-UV-absorbing compounds in HPLC?

4. 
Which type of chromatography would you use to separate proteins based on their size?

5. 
What is the primary advantage of using gradient elution in HPLC over isocratic elution?

6. 
Which type of interaction is primarily responsible for the separation in normal-phase chromatography?

7. 
In gas chromatography, which of the following factors primarily determines the retention time of a compound?

8. 
What is the purpose of using an internal standard in quantitative chromatography?

9. 
In reversed-phase HPLC, what is the typical nature of the stationary phase?

10. 
Which of the following is a common mobile phase used in supercritical fluid chromatography?

11. 
How does increasing the particle size of the stationary phase affect the efficiency of separation in chromatography?

12. 
Which of the following modifications in a gas chromatography setup would most likely improve the separation of two closely eluting peaks?

13. 
In ion-exchange chromatography, what is the effect of increasing the ionic strength of the mobile phase on the retention time of the analytes?

14. 
What is the main reason for using a guard column in HPLC?

15. 
In affinity chromatography, how is the specific interaction between the target molecule and the stationary phase achieved?

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