Southern blotting is a molecular biology technique used to detect specific DNA sequences in a sample. It involves digesting DNA, electrophoresis, transferring the DNA to a membrane, and then using a labeled probe to hybridize and identify the target DNA sequence. This technique is named after its inventor, Edwin Southern. […]
pBR322 is a well-known plasmid DNA used in molecular biology and biotechnology. It was one of the first plasmids developed for cloning and genetic engineering. pBR322 contains genes for antibiotic resistance and multiple cloning sites, making it a valuable tool for DNA manipulation and recombinant DNA studies. This is an […]
A TI (tumour-inducing) plasmid is a type of circular DNA molecule found in certain strains of the bacterium Agrobacterium tumefaciens. It plays a crucial role in the bacterium’s ability to infect plants and transfer genes into them, causing the formation of crown gall tumours. TI plasmids have been extensively studied […]
Homology modelling, also known as comparative modelling, is a computational technique in structural biology. It predicts the 3D structure of a protein based on the known structure of a related protein (homolog). It relies on the assumption that structurally similar proteins share similar functions. This is an MCQ on Homology […]
Genetic markers are specific DNA sequences or variations that serve as identifiable points on a person’s or organism’s genome. They are used in genetic research, diagnostics, and ancestry tracing to identify genetic traits, mutations, or associations with diseases, providing valuable insights into an individual’s or population’s genetics. This is an […]