MCQ on DNA Polymerase with Answers (PDF)

DNA polymerase is an enzyme responsible for synthesizing new DNA strands during replication by adding nucleotides to a pre-existing strand using the original DNA as a template. It works in the 5′ to 3′ direction, ensuring accurate base-pairing to maintain genetic fidelity. DNA polymerase also has proofreading capabilities, correcting errors during replication to minimize mutations. This enzyme is crucial for cell division, enabling the transmission of genetic information to daughter cells. This is an MCQ on DNA Polymerase with Answers. You can download all these questions as PDF from the results page of this quiz.

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1. 
Which of the following activities is NOT associated with DNA polymerase?

2. 
What is the primary function of the 3' to 5' exonuclease activity of DNA polymerase?

3. 
Which DNA polymerase is primarily responsible for synthesizing the leading strand during DNA replication in eukaryotes?

4. 
Which DNA polymerase has the highest fidelity and processivity in eukaryotic cells?

5. 
What feature of DNA polymerase allows it to add nucleotides to the 3' end of a growing DNA strand?

6. 
Which DNA polymerase is primarily involved in DNA repair in eukaryotic cells?

7. 
How does DNA polymerase ensure accurate DNA replication?

8. 
What is the role of DNA polymerase α (alpha) in eukaryotic DNA replication?

9. 
Which DNA polymerase is involved in mitochondrial DNA replication?

10. 
What is the primary function of the sliding clamp in DNA replication?

11. 
Which property of DNA polymerase limits it to adding nucleotides only to the 3' end of the growing DNA strand?

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