MCQ on Restriction Enzymes with Answers (PDF)

A restriction enzyme, or restriction endonuclease, is a protein produced by bacteria that cuts DNA at specific sequences, known as recognition sites. These enzymes serve as a defense mechanism against invading viruses by chopping up foreign DNA. In biotechnology, restriction enzymes are used for genetic engineering, as they allow precise cutting and manipulation of DNA for cloning, sequencing, and analysis, forming the basis of recombinant DNA technology. This is an MCQ on Restriction Enzymes with Answer Key. You can download the PDF of this question paper from the download link provided in the results page of this quiz.

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1. 
What is the primary function of restriction enzymes in bacteria?

2. 
Which of the following best describes the type of cut made by Type II restriction enzymes?

3. 
Which of the following enzymes is commonly used for cloning applications due to its ability to produce compatible sticky ends?

4. 
What characteristic of Type I restriction enzymes distinguishes them from Type II restriction enzymes?

5. 
What is the significance of restriction enzyme recognition sites being palindromic?

6. 
In the context of restriction enzymes, what does the term “isoschizomer” refer to?

7. 
Which type of restriction enzyme is used for creating blunt-end cuts in DNA? EcoRI

8. 
How does DNA methylation affect the activity of restriction enzymes?

9. 
What is the primary use of restriction enzymes in recombinant DNA technology?

10. 
Which of the following methods is used to separate DNA fragments generated by restriction enzymes?

11. 
What is the advantage of using restriction enzymes with sticky ends over those with blunt ends?

12. 
Which property of restriction enzymes is crucial for their application in constructing recombinant DNA molecules?

13. 
What is the role of a “restriction map” in molecular biology?

14. 
Which technique can be used to identify specific restriction enzyme cut sites in a DNA sequence?

15. 
What impact does the presence of restriction enzyme cut sites in a gene have on gene cloning?

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