Northern blotting is a molecular biology technique used to study gene expression by detecting and analyzing specific RNA molecules in a sample. It involves electrophoresis to separate RNA fragments by size and hybridization with a labeled probe to identify target RNA sequences. This is an MCQ on Northern Blotting Technique […]
Electrophoretic Mobility Shift Assay (EMSA), also known as gel shift or band shift assay, is a molecular biology technique. It’s used to study protein–DNA interactions by observing changes in the mobility of DNA fragments in an electrophoresis gel when proteins bind to them, helping analyze DNA-protein interactions. This is an […]
Enzyme-Linked Immunosorbent Assay (ELISA) is a biochemical test used to detect and quantify specific proteins, antibodies, or antigens in biological samples. It involves using antibodies and enzymes to create a color change that indicates the presence and concentration of the target molecule, making it valuable in various research and diagnostic […]
The CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated proteins) system is a revolutionary genome editing tool in molecular biology. It enables precise modification of DNA sequences in living organisms, allowing for gene insertion, deletion, or replacement. Cas proteins, guided by RNA molecules, target specific DNA sequences for modification, […]
Cre-lox recombination is a genetic engineering technique used to manipulate DNA in living organisms. It involves the enzyme Cre recombinase, which recognizes specific DNA sequences (loxP sites) to excise, insert, or invert genetic segments. It is valuable for conditional gene knockout and controlled gene expression in research and biotechnology. This […]