Western blotting is a laboratory technique used to detect and analyze proteins. It involves separating proteins based on size through gel electrophoresis, transferring them to a membrane, and then using specific antibodies to visualize and identify the target proteins. This is an MCQ on Western Blotting – Principles and Applications […]
Immunofluorescence is a laboratory technique that utilizes fluorescently labeled antibodies to detect and visualize specific proteins or antigens within cells or tissues. When exposed to light of a particular wavelength, the labeled antibodies emit fluorescence, enabling precise localization and identification of target molecules in biological samples. This is an MCQ […]
Green fluorescent proteins (GFP) are naturally occurring proteins that emit green light when exposed to ultraviolet or blue light. Widely used as a biomarker, GFP can be genetically fused to other proteins to visualize and track their location and movement within living cells, aiding in biological research and imaging. This […]
Gene knockout is a genetic technique wherein a specific gene is intentionally deactivated or “knocked out” in an organism. This allows researchers to study the effects of gene loss, revealing insights into gene function and its role in biological processes. This is an MCQ on Gene Knockout Technology- Principles and […]
Northern blotting is a molecular biology technique used to study gene expression by detecting and analyzing specific RNA molecules in a sample. It involves electrophoresis to separate RNA fragments by size and hybridization with a labeled probe to identify target RNA sequences. This is an MCQ on Northern Blotting Technique […]