Next-generation sequencing (NGS) encompasses advanced DNA sequencing technologies capable of high-throughput, parallel sequencing of millions of DNA fragments. This approach revolutionizes genomic research by offering rapid, cost-effective analyses, enabling various applications such as whole genome sequencing, transcriptomics, and metagenomics. This is an MCQ on Next Generation Sequencing Principle and Applications. […]

Shotgun sequencing is a method to sequence DNA by randomly breaking it into smaller fragments, sequencing them, and then assembling the overlapping sequences. This approach enables rapid determination of the entire genome, facilitating genomic research and genome assembly. This is an MCQ on Shotgun Sequencing Methodology of DNA Sequencing for […]

Pyrosequencing is a DNA sequencing technique that relies on the detection of released pyrophosphate upon nucleotide incorporation. It involves a series of enzymatic reactions, generating light proportional to the number of incorporated nucleotides, allowing for accurate sequence determination. This is an MCQ on Pyrosequencing of DNA- Principles and Applications. |  […]

Illumina sequencing, a widely used method, employs fluorescently labeled nucleotides and reversible termination to sequence DNA fragments. Through iterative cycles of nucleotide incorporation and imaging, it accurately determines the sequence, offering high throughput, speed, and cost-effectiveness, revolutionizing genomic research and applications. This is an MCQ on Illumina Sequencing Technique of […]