Crossing over is a genetic process during meiosis where homologous chromosomes exchange segments of DNA. This exchange results in genetic variation among offspring by creating new combinations of genes, enhancing diversity, and contributing to evolution’s mechanism. This is an MCQ on Linkage and Crossing Over – Mechanism and its Significance […]
Radioimmunoassay (RIA) is a sensitive laboratory technique that uses radioactive isotopes to measure the concentration of specific substances, such as hormones or antigens, in biological samples. It involves competition between labeled and unlabeled molecules for binding to a limited number of specific antibodies. This is an MCQ on RIA (Radioimmunoassay)- […]
Western blotting is a laboratory technique used to detect and analyze proteins. It involves separating proteins based on size through gel electrophoresis, transferring them to a membrane, and then using specific antibodies to visualize and identify the target proteins. This is an MCQ on Western Blotting – Principles and Applications […]
Immunofluorescence is a laboratory technique that utilizes fluorescently labeled antibodies to detect and visualize specific proteins or antigens within cells or tissues. When exposed to light of a particular wavelength, the labeled antibodies emit fluorescence, enabling precise localization and identification of target molecules in biological samples. This is an MCQ […]
Green fluorescent proteins (GFP) are naturally occurring proteins that emit green light when exposed to ultraviolet or blue light. Widely used as a biomarker, GFP can be genetically fused to other proteins to visualize and track their location and movement within living cells, aiding in biological research and imaging. This […]
Gene knockout is a genetic technique wherein a specific gene is intentionally deactivated or “knocked out” in an organism. This allows researchers to study the effects of gene loss, revealing insights into gene function and its role in biological processes. This is an MCQ on Gene Knockout Technology- Principles and […]
Northern blotting is a molecular biology technique used to study gene expression by detecting and analyzing specific RNA molecules in a sample. It involves electrophoresis to separate RNA fragments by size and hybridization with a labeled probe to identify target RNA sequences. This is an MCQ on Northern Blotting Technique […]
Electrophoretic Mobility Shift Assay (EMSA), also known as gel shift or band shift assay, is a molecular biology technique. It’s used to study protein–DNA interactions by observing changes in the mobility of DNA fragments in an electrophoresis gel when proteins bind to them, helping analyze DNA-protein interactions. This is an […]
Enzyme-Linked Immunosorbent Assay (ELISA) is a biochemical test used to detect and quantify specific proteins, antibodies, or antigens in biological samples. It involves using antibodies and enzymes to create a color change that indicates the presence and concentration of the target molecule, making it valuable in various research and diagnostic […]