Bacterial conjugation is a process where one bacterium transfers genetic material to another through direct contact. A donor cell with a plasmid forms a pilus to connect with a recipient cell, transferring genetic material that can lead to new traits or antibiotic resistance in the recipient. This is an MCQ […]
A restriction enzyme, or restriction endonuclease, is a protein produced by bacteria that cuts DNA at specific sequences, known as recognition sites. These enzymes serve as a defense mechanism against invading viruses by chopping up foreign DNA. In biotechnology, restriction enzymes are used for genetic engineering, as they allow precise […]
Restriction endonucleases, or restriction enzymes, are proteins that cut DNA at specific recognition sequences, usually palindromic. Found naturally in bacteria, they serve as a defense mechanism by cleaving foreign DNA, such as that of viruses. Each enzyme recognizes a particular sequence of nucleotides and cuts at or near this site, […]
Chromatography is a laboratory technique used to separate, identify, and analyze components in a mixture. It involves a stationary phase and a mobile phase. As the mixture moves through the stationary phase, different components travel at different rates, leading to separation. Types include gas, liquid, paper, and thin-layer chromatography, each […]
Column chromatography is a technique used to separate and purify individual compounds from a mixture. It involves a column filled with a stationary phase (usually silica gel or alumina) through which the mixture is passed along with a mobile phase (a solvent or solvent mixture). As the mixture moves through […]