MCQs on TI Plasmid and its Applications in Biotechnology

A TI (tumour-inducing) plasmid is a type of circular DNA molecule found in certain strains of the bacterium Agrobacterium tumefaciens. It plays a crucial role in the bacterium’s ability to infect plants and transfer genes into them, causing the formation of crown gall tumours. TI plasmids have been extensively studied and utilized in biotechnology for plant genetic engineering. This is an MCQ on TI Plasmid and its applications in biotechnology an genetic engineering.

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1. 
The Ti (Tumor-inducing) plasmid is commonly found in which type of bacteria?

2. 
The Ti plasmid is known for its ability to cause crown gall disease in which type of plant?

3. 
The Ti plasmid contains specific DNA regions known as T-DNA. What is the role of T-DNA in genetic engineering?

4. 
Which enzyme is responsible for the transfer of T-DNA from the Ti plasmid into the plant cell?

5. 
The process of transferring genes of interest into plants using the Ti plasmid is commonly known as:

6. 
In genetic engineering, the genes of interest are inserted into the Ti plasmid using recombinant DNA technology. What is the resulting modified Ti plasmid called?

7. 
Which of the following is NOT a common application of the Ti plasmid in genetic engineering?

8. 
The Ti plasmid is used to create genetically modified crops. Which trait can be introduced into crops using this technology to improve their agricultural performance?

9. 
The Ti plasmid-based genetic engineering has been used to produce "Golden Rice." What is the special characteristic of Golden Rice?

10. 
The process of introducing Ti plasmid into plant cells usually involves which technique to deliver the DNA into the plant?

11. 
The Ti plasmid-based transformation is widely used for crop improvement. However, it is primarily applicable to:

12. 
The genes of interest introduced into the Ti plasmid are often driven by specific regulatory sequences to control their expression. What are these sequences called?

13. 
In addition to plants, the Ti plasmid can be used to transfer genes into which other types of organisms?

14. 
The Ti plasmid has revolutionized agriculture and biotechnology due to its ability to transfer genes efficiently. Which scientific breakthrough led to the discovery and application of the Ti plasmid in genetic engineering?

15. 
The use of the Ti plasmid in genetic engineering has raised concerns about potential ecological and environmental impacts. What is one of the main concerns associated with the release of genetically modified plants into the environment?

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