MCQ on RT PCR with Answers

Reverse Transcription Polymerase Chain Reaction (RT-PCR) is a molecular biology technique used to detect and quantify RNA. It involves reverse transcription of RNA into complementary DNA (cDNA) by reverse transcriptase, followed by amplification using PCR. RT-PCR is highly sensitive and specific, allowing the analysis of gene expression, detection of RNA viruses, and other RNA-based studies. This technique can be performed in one-step or two-step formats, with various modifications to suit specific experimental needs. It plays a significant role in research, diagnostics, and biotechnology, offering a robust method to study RNA molecules with precision. This is an MCQ on MCQ on RT PCR with Answers.

Molecular Biology Notes  |  Molecular Biology PPTs  |  Molecular Biology MCQs

1. 
Which enzyme is responsible for converting RNA into cDNA during RT-PCR?

2. 
What is the primary function of primers in RT-PCR?

3. 
Which type of RT-PCR uses fluorescent dyes or probes for real-time quantification?

4. 
In a two-step RT-PCR process, what is performed in the first step?

5. 
What is the primary role of Taq polymerase in RT-PCR?

6. 
Which of the following is a characteristic of a one-step RT-PCR?

7. 
What is the purpose of a melting curve analysis in RT-PCR?

8. 
Which of the following represents the annealing phase in RT-PCR?

9. 
What is a key advantage of SYBR Green dye in RT-PCR?

10. 
Which factor can affect the efficiency of RT-PCR?

11. 
In digital RT-PCR, what is the primary advantage?

12. 
What is the optimal temperature range for the reverse transcription step in RT-PCR?

13. 
How is RNA contamination avoided during RT-PCR?

14. 
What is the purpose of normalization in RT-PCR data analysis?

15. 
Which of the following is a common challenge in RT-PCR?

<<< Back to Molecular Biology MCQ Page

More Multiple Choice Questions (MCQs)...
BOTANYBIOCHEMISTRYBIOTECHNOLOGY
ZOOLOGYCELL BIOLOGYIMMUNOLOGY
ECOLOGYMOLECULAR BIOLOGYANIMAL PHYSIOLOGY
GENETICSBIOINFORMATICSPLANT PHYSIOLOGY
EVOLUTIONBIOPHYSICSDEVELOPMENTAL BIOLOGY
MICROBIOLOGYBIOSTATISTICSRESEARCH METHODOLOGY

MCQ on RT PCR




You may also like...
NOTESQUESTION BANKCOMPETITIVE EXAMS.
PPTsUNIVERSITY EXAMSDIFFERENCE BETWEEN..
MCQsPLUS ONE BIOLOGYNEWS & JOBS
MOCK TESTSPLUS TWO BIOLOGYPRACTICAL

Related posts:

  1. MCQ on Helix-Loop-Helix (HLH) Domain
  2. MCQ on Histone: Organization and Functions
  3. MCQ on DNA Replication in Eukaryotes
  4. MCQ on Molecular Chaperones
  5. MCQ on Molecular Mechanism of Mutation with Answers (PDF)

Leave a Reply

Your email address will not be published. Required fields are marked *