MCQ on Cloning Vectors with Answers (PDF)

Cloning vectors are DNA molecules used to carry and replicate a specific gene or DNA fragment in a host cell. They are essential tools in gene cloning. Common types include plasmids, bacteriophages, and cosmids. Vectors have features like an origin of replication (to multiply within the host), selectable markers (for identifying cells that contain the vector), and multiple cloning sites (for inserting foreign DNA). Cloning vectors allow scientists to produce large quantities of a gene for research or biotechnology applications. This is an MCQ on Cloning Vectors with Answers. You can download the questions as PDF from the result window of this quiz.

Biotechnology Notes  |  Biotechnology PPTs  |  Biotechnology MCQs

1. 
Which feature of a cloning vector ensures it can replicate independently in a host cell?

2. 
Which of the following is an example of a commonly used plasmid cloning vector?

3. 
Which cloning vector is most suitable for cloning large fragments of DNA, typically over 100 kb?

4. 
What is the purpose of a selectable marker in a cloning vector?

5. 
Which of the following is a bacteriophage-based cloning vector?

6. 
Which feature of a plasmid vector allows for easy insertion of foreign DNA?

7. 
Which of the following is a yeast-based cloning vector?

8. 
What is the role of the "ampicillin resistance" gene in cloning vectors?

9. 
What is a cosmid vector?

10. 
What is the main purpose of the "lacZ" gene in cloning vectors?

11. 
Which vector is typically used for cloning in plant cells?

12. 
Which of the following is a feature of plasmid vectors that ensures a high copy number in bacterial cells?

13. 
What is the typical size of DNA fragments that can be cloned using cosmids?

14. 
Which vector is suitable for cloning in mammalian cells?

15. 
What is the significance of a high copy number vector?

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