MCQ on BLAST (Basic Local Alignment Search Tool)

BLAST (Basic Local Alignment Search Tool) is a widely used bioinformatics tool developed by the National Institutes of Health (NIH) for comparing biological sequences against databases to identify homologous sequences, infer evolutionary relationships, and predict functional annotations. This is an MCQ on BLAST Analysis: Principles and Applications in Bioinformatics and Computational Biology.

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1. 
Which organization developed the Basic Local Alignment Search Tool (BLAST)?

2. 
BLAST primarily searches databases for what type of biological sequences?

3. 
What does the term "local" signify in BLAST?

4. 
Which BLAST program is suitable for comparing a query sequence against a protein sequence database?

5. 
What does the E-value in BLAST results represent?

6. 
In BLAST, what is the purpose of the "word size" parameter?

7. 
Which BLAST program is used to compare a translated nucleotide query sequence against a protein sequence database?

8. 
What does the "Query Coverage" statistic in BLAST results indicate?

9. 
Which BLAST program is used for comparing a protein query sequence against a translated nucleotide database?

10. 
In BLAST, what does the "Bit Score" represent?

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MCQ on BLAST Analysis

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