Advanced Biochemistry MCQ-21 (PG Level)

The reaction of glutamate and ammonia to glutamine and water has a value of +14 kJ mol^-1 for ΔG⁰. This is coupled with the ATP reaction (ATP + H2) ⇄ ADP + Phosphate). The ΔG⁰ for this reaction I -30 kJ mol^-1. The ΔG⁰ (kJ mol^-1) for the coupled reaction Glutamate + NH3 + ATP ⇄ Glutamine + ADP + Phosphate under equilibrium condition is: (CSIR_2015_I)

A protein is composed of leucine, isoleucine, alanine, glycine, proline, one lysine, one arginine and two cysteines connected by a disulfide bond. Conformational analysis indicates that the protein has elements of helix and beta structure. The protein is most likely: (CSIR_2015_I)

You are studying a protein that inserts itself into a model membrane (liposomes) during a reconstitution process. The protein has an N-terminal, 18 amino acid hydrophilic segment that is located on the outside of the membrane, a 19 amino acid hydrophobic trans membrane segment flanked by negatively and positively charged amino acids, and a C terminal domain that resides inside the lumen (a depicted below in the diagram).

For proper reconstitution of the protein, which of the following strategies will be appropriate? (CSIR_2015_I)

Which one of the following would contribute to intrinsic fluorescence to a protein? (CSIR_2015_II)

Which one of the following statements is correct? (CSIR_2015_II)

Four single amino acid mutants (a to d) of a protein in the epitope-region of a monoclonal antibody X were made and expressed in E. coli. The lysates from the four E. coli cultures expressing these four proteins were run or an SDS-PAGE gel and subsequently transferred to nitrocellulose membrane and Western blotted using a monoclonal antibody X raised against the wild type protein. The results are presented in the figure below

The four single mutations, upon sequencing, were found to be Valine (V) to Alanine (A); Glycine (G) to Proline (P); Alanine (A) to Aspartic acid (D) and isoleucine (I) to leucine (L).

Which one of the following statements is correct? (CSIR_2015_II)

The di- and tripeptides are transported in the enterocytes by peptide transporter 1 that requires:- (CSIR_2016_I)

The standard free energy change (ΔG0) per mole for the reaction A ⇄ B at 30⁰C in an open system is -1000 cal/mole. What is the approximate free energy change (ΔG) when the concentration of A and B are 100 micromolar and 100 millimolar, respectively? (CSIR_2016_I)

The turnover number and specific activity of an enzyme ( molecular weight 40,000 D) in a reaction (Vmax= 4 μ mol of substrate reacted/ min, enzyme amount = 2 μ g) are: (CSIR_2016_I)

E. coli was grown in three different experimental conditions. In one, it was grown in medium containing glucose as carbon source; in the second in medium containing both glucose and galactose; and in third was infected with phage. Match the curves shown below to the treatment: (CSIR_2016_I)